Macrobrachium rosenbergii microsporidia visualized quick detection kit and detection method thereof

一种罗氏沼虾微胞子虫可视化快速检测试剂盒及其检测方法

Abstract

本发明公开了一种罗氏沼虾微胞子虫可视化快速检测试剂盒及其检测方法,其中,罗氏沼虾微胞子虫可视化快速检测试剂盒包括微胞子虫DNA提取试剂和反应试剂,所述的反应试剂中含有用于检测罗氏沼虾微胞子虫扩增用核酸引物组,该引物组包含引物MrMSP‑F3、引物MrMSP‑B3、引物MrMSP‑FIP、引物MrMSP‑BIP、引物MrMSP‑LpF和引物MrMSP‑LpB;本发明的快速诊断试剂盒建立了一套经过优化的快速扩增反应体系,不但使得罗氏沼虾微胞子虫定性检测更加简便快速、特异性高、灵敏度高,而且该试剂盒可用于现场检测,具有很高的科研和经济价值。
The invention discloses a macrobrachium rosenbergii microsporidia visualized quick detection kit and a detection method thereof. The macrobrachium rosenbergii microsporidia visualized quick detection kit comprises a microsporidia DNA extraction reagent and a reaction reagent, and the reaction reagent contains a nucleic acid primer set used for detecting macrobrachium rosenbergii microsporidia proliferation, wherein a primer group comprises a primer MrMSP-F3, a primer MrMSP-B3, a primer MrMSP-FIP, a primer MrMSP-BIP, a primer MrMSP-LpF and a primer MrMSP-LpB. According to the macrobrachium rosenbergii microsporidia visualized quick detection kit, a set of optimized quick proliferation reaction system is established, so that not only is macrobrachium rosenbergii microsporidia qualitative detection simpler and faster, high in specificity and high in sensitivity, but also the kit can be used for field detection, and has very high scientific study and economic value.

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